Culturing Stem Cell Awareness
نویسندگان
چکیده
منابع مشابه
Effect of Different Concentrations of Forskolin Along with Mature Granulosa Cell Co-Culturing on Mouse Embryonic Stem Cell Differentiation into Germ-Like Cells
Background: Germ cell development processes are influenced by soluble factors and intercellular signaling events between them and the neighboring somatic cells. More insight into the molecular biology of the germ cell development from embryonic stem (ES) cells and investigation of appropriate factors, specifically those targeting differentiation process, is of great importance. In this study, w...
متن کاملFrom Nano to Macro: Multiscale Materials for Improved Stem Cell Culturing and Analysis.
Stem cells respond to nanoscale, microscale, and macroscale cues, such as matrix, growth factors, and niche organization, which are difficult to physiologically recapitulate in culture. We discuss how utilizing bioengineering approaches to manipulate and integrate spatiotemporal cues across these discrete length scales can improve traditional methods for controlling cell fate.
متن کاملA Comparative Study of Protocols for Mouse Embryonic Stem Cell Culturing
Most stem cell laboratories still rely on old culture methods to support the expansion and maintenance of mouse embryonic stem (ES) cells. These involve growing cells on mouse embryonic fibroblast feeder cells or on gelatin in media supplemented with fetal bovine serum and leukemia inhibitory factor (LIF). However, these techniques have several drawbacks including the need for feeder-cells and/...
متن کاملCulturing on Wharton's Jelly Extract Delays Mesenchymal Stem Cell Senescence through p53 and p16INK4a/pRb Pathways
Mesenchymal stem cells (MSCs) hold great therapeutic potential. However, MSCs undergo replication senescence during the in vitro expansion process. Wharton's jelly from the human umbilical cord harbors a large number of MSCs. In this study, we hypothesized that Wharton's jelly would be beneficial for in vitro expansion of MSCs. Wharton's jelly extract (WJEs), which is mainly composed of extrace...
متن کاملCulturing intestinal stem cells: applications for colorectal cancer research
Recent advance of sequencing technology has revealed genetic alterations in colorectal cancer (CRC). The biological function of recurrently mutated genes has been intensively investigated through mouse genetic models and CRC cell lines. Although these experimental models may not fully reflect biological traits of human intestinal epithelium, they provided insights into the understanding of inte...
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ژورنال
عنوان ژورنال: Cell Stem Cell
سال: 2009
ISSN: 1934-5909
DOI: 10.1016/j.stem.2009.10.001